Effects of Storage Time and Exogenous Protease Inhibitors on Plasma Protein Levels Ayache et. al. american Journal of Clinical Pathology, 2006 vol. 126 (2) pp. 174-184.
This is an elegant study demonstrating the significant changes in a wide variety (~37) of biomarkers with a 2-hour hold prior to processing. The study also evaluates the influence of protease inhibitors sample behavior. Why we like this article: Beyond the issues of handling and short-term storage of plasma, the study provoked an excited discussion amongst BioCoR facutly who could see the implications of the study extending far beyond the conclusions made in the paper.
Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions
Published on: Wednesday 23, June 2010Category: article of interest
Cao et al. Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnol. Bioeng. (2003) vol. 82 (6) pp. 684-90
The article clearly describes the importance of both cooling and thawing rates on the post thaw activity of proteins.
Storage conditions influence post thaw recovery
Published on: Thursday 11, February 2010Category: article of interest
Fowke, K.R., et al., Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells. J Immunol Methods, 2000, 244(1-2): p. 139-44.
The article compares the influence of storage temperature on recovery of human whole blood samples.
Pathways of post thaw apoptosis
Published on: Wednesday 10, February 2010Category: article of interest
Stroh, C., et al., The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells. Faseb J, 2002, ,16(12): p.
Novel cryoprotective agents
Published on: Tuesday 09, February 2010Category: article of interest
Matsumura, K. and S.H. Hyon, Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties. Biomaterials, 2009, 30(27): p.
Bag breakage at low temperature
Published on: Monday 08, February 2010Category: article of interest
Khuu, H.M., et al., Catastrophic failures of freezing bags of cellular therapy products: description, case, and consequences. Cytotherapy, 2002. 4(6): p.
Effects of Storage Time and Exogenous Protease Inhibitors on Plasma Protein Levels
Published on: Tuesday 26, January 2010Category: articles of interet
Effects of Storage Time and Exogenous Protease Inhibitors on Plasma Protein Levels Ayache et. al. american Journal of Clinical Pathology, 2006 vol. 126 (2) pp.
Review of stem cell cryopreservation
Published on: Monday 25, January 2010Category: article of interest
A review of current methods of preserving hematopoietic, mesenchymal and embryonic stem cells is presented. Emerging issues for preserving stem cells for therapeutic as well as research purposes are presented.
